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Other_recombinant_protein >
ubpbio/K48 polyUb Chain-binding Protein Identification / Validation Kit/J2500/1 Kit
TheNon-cleavableK48polyUbchain(2-4)areresistantofdeubiquitinationwhenincubatingwithwholecellortissuelysates,thusallowingmaximallycaptureproteinsthatspecificallybindK48polyUbchains.Afterbinding,6xHis-Non-cleavableK48polyUbchainsandthebindingproteinscanbeenrichedbyNickelXPureAgaroseResin(included).Theboundproteinsandthenon-cleavableK48polyUbchainscanbeelutedfromNiresinusingabuffercontaining250mMimidazole(included)orbyincubatingwiththrombin(notincluded).Eitheroftheelutionmethodcansignificantlyreducetheamountsofnon-specificbindingproteinswhencomparedwithelutionusingSDSsamplebuffer.
AdditionalInformation
| ProductName: | K48polyUbChain-bindingProteinIdentification/ValidationKit |
|---|---|
| AlsoKnownAs: | N/A |
| CatalogNo.: | J2500 |
| Size: | 1Kit |
| MolecularWeight: | N/A |
| Species: | Human |
| Source: | Bacterialrecombinant |
| Stock: | 20mMTris,150mMNaCl,2mMβME,10%Glycerol |
| Concentration: | Seetubelabel |
| QualityAssurance: | 95%basedonSDS-PAGE |
| Storage: | Seethestorageconditiononthelabelofeachcomponent;avoidmultiplefreeze-thawcycles |
| PDFDataSheet: | PDFdatasheet |
| NCBIRefSeq: | N/A |
| Image(s): | No |
| ShippingMethod: | Dryiceshipping |
| References: | N/A |

