>
产品中心 >
Other_recombinant_protein >
ubpbio/K48 polyUb Chain-binding Protein Identification / Validation Kit/J2500/1 Kit

TheNon-cleavableK48polyUbchain(2-4)areresistantofdeubiquitinationwhenincubatingwithwholecellortissuelysates,thusallowingmaximallycaptureproteinsthatspecificallybindK48polyUbchains.Afterbinding,6xHis-Non-cleavableK48polyUbchainsandthebindingproteinscanbeenrichedbyNickelXPureAgaroseResin(included).Theboundproteinsandthenon-cleavableK48polyUbchainscanbeelutedfromNiresinusingabuffercontaining250mMimidazole(included)orbyincubatingwiththrombin(notincluded).Eitheroftheelutionmethodcansignificantlyreducetheamountsofnon-specificbindingproteinswhencomparedwithelutionusingSDSsamplebuffer.
AdditionalInformation
ProductName: | K48polyUbChain-bindingProteinIdentification/ValidationKit |
---|---|
AlsoKnownAs: | N/A |
CatalogNo.: | J2500 |
Size: | 1Kit |
MolecularWeight: | N/A |
Species: | Human |
Source: | Bacterialrecombinant |
Stock: | 20mMTris,150mMNaCl,2mMβME,10%Glycerol |
Concentration: | Seetubelabel |
QualityAssurance: | 95%basedonSDS-PAGE |
Storage: | Seethestorageconditiononthelabelofeachcomponent;avoidmultiplefreeze-thawcycles |
PDFDataSheet: | PDFdatasheet |
NCBIRefSeq: | N/A |
Image(s): | No |
ShippingMethod: | Dryiceshipping |
References: | N/A |