ubpbio/6xHis-TEV蛋白酶/P1001-200/200µg
市场价:
¥2700.00
美元价:
1620.00
产品分类:
其它酶
公司分类:
Other_enzymes
联系Q Q:
3392242852
电话号码:
4000-520-616
电子邮箱:
info@ebiomall.com
商品介绍
TobaccoEtchVirus(TEV)proteaseisacysteineproteasethatiscommonlyusedtoremovefusiontags,includingHis,GSTandMBP,attheNorCterminiofrecombinantproteins.TEVproteasespecificallyrecognizesasevenaminoacidsequenceGlu-Asn-Leu-Tyr-Phe-Gln-Gly/Ser(ENLYFQG/S)andcleavesbetweenGlnandGly/Serunderawiderangeofconditions.
Recombinant6xHis-TEVwasexpressedinE.Coliandpurifiedtonearhomogeneity.ASer219Asn(S219N)substitutionwasintroducedthathasnoeffectonTEVproteaseactivity,butinhibitsauto-cleavageandthus,stABIlizingTEVproteaseduringincubationandlong-termstorage.
Werecommendusing3%suppliedTEVprotease(w/w)topreformreactionsat40Cfor12-16horat300Cfor1-4h.Dependingonspecificsubstrates,TEVproteaseconcentration,incubationtime,andreactiontemperatureshouldbeoptimized.6xHis-TEVcanberemovedbyNi-NTAresinafterincubation.
TEVproteasemaintainshighactivityunderawiderangeoftemperatures(40C-300C),pH(6.0-8.5)andbuffers,andinthepresenceofcommonlyusedproteaseinhibitors.
Recombinant6xHis-TEVwasexpressedinE.Coliandpurifiedtonearhomogeneity.ASer219Asn(S219N)substitutionwasintroducedthathasnoeffectonTEVproteaseactivity,butinhibitsauto-cleavageandthus,stABIlizingTEVproteaseduringincubationandlong-termstorage.
Werecommendusing3%suppliedTEVprotease(w/w)topreformreactionsat40Cfor12-16horat300Cfor1-4h.Dependingonspecificsubstrates,TEVproteaseconcentration,incubationtime,andreactiontemperatureshouldbeoptimized.6xHis-TEVcanberemovedbyNi-NTAresinafterincubation.
TEVproteasemaintainshighactivityunderawiderangeoftemperatures(40C-300C),pH(6.0-8.5)andbuffers,andinthepresenceofcommonlyusedproteaseinhibitors.
AdditionalInformation
ProductName: | 6xHis-TEVProtease |
---|---|
AlsoKnownAs: | TEVprotease,NIa,Tobaccoetchvirusprotease |
CatalogNo.: | P1001-200 |
Size: | 200µg |
MolecularWeight: | 27kDa |
Species: | TabaccoEtchVirus |
Source: | Bacterialrecombinant |
Stock: | 100mMTris,pH8.5at40C,500mMNaCl,5mMDTT,0.5mMEDTAand50%Glycerol |
Concentration: | Seetubelabel |
QualityAssurance: | >90%puritybasedonSDS-PAGE. |
Storage: | Storeat-80°C;avoidmultiplefreeze-thawcycles |
PDFDataSheet: | PDFDatasheet,MSDS |
NCBIRefSeq: | N/A |
Image(s): | |
ShippingMethod: | Dryiceshipping |
References: | 1.LucastLJ.,etal.(2001)Biotechniques30,544. |