ubpbio/6xHis-TEV Protease/P1001-10000/10 X 1 mg
市场价:
¥39900.00
美元价:
23940.00
产品分类:
其它酶
公司分类:
Other_enzymes
联系Q Q:
3392242852
电话号码:
4000-520-616
电子邮箱:
info@ebiomall.com
商品介绍
TobaccoEtchVirus(TEV)proteaseisacysteineproteasethatiscommonlyusedtoremovefusiontags,includingHis,GSTandMBP,attheNorCterminiofrecombinantproteins.TEVproteasespecificallyrecognizesasevenaminoacidsequenceGlu-Asn-Leu-Tyr-Phe-Gln-Gly/Ser(ENLYFQG/S)andcleavesbetweenGlnandGly/Serunderawiderangeofconditions.
Recombinant6xHis-TEVwasexpressedinE.Coliandpurifiedtonearhomogeneity.ASer219Asn(S219N)substitutionwasintroducedthathasnoeffectonTEVproteaseactivity,butinhibitsauto-cleavageandthus,stABIlizingTEVproteaseduringincubationandlong-termstorage.
Werecommendusing3%suppliedTEVprotease(w/w)topreformreactionsat40Cfor12-16horat300Cfor1-4h.Dependingonspecificsubstrates,TEVproteaseconcentration,incubationtime,andreactiontemperatureshouldbeoptimized.6xHis-TEVcanberemovedbyNi-NTAresinafterincubation.
TEVproteasemaintainshighactivityunderawiderangeoftemperatures(40C-300C),pH(6.0-8.5)andbuffers,andinthepresenceofcommonlyusedproteaseinhibitors.
Recombinant6xHis-TEVwasexpressedinE.Coliandpurifiedtonearhomogeneity.ASer219Asn(S219N)substitutionwasintroducedthathasnoeffectonTEVproteaseactivity,butinhibitsauto-cleavageandthus,stABIlizingTEVproteaseduringincubationandlong-termstorage.
Werecommendusing3%suppliedTEVprotease(w/w)topreformreactionsat40Cfor12-16horat300Cfor1-4h.Dependingonspecificsubstrates,TEVproteaseconcentration,incubationtime,andreactiontemperatureshouldbeoptimized.6xHis-TEVcanberemovedbyNi-NTAresinafterincubation.
TEVproteasemaintainshighactivityunderawiderangeoftemperatures(40C-300C),pH(6.0-8.5)andbuffers,andinthepresenceofcommonlyusedproteaseinhibitors.
AdditionalInformation
| ProductName: | 6xHis-TEVProtease |
|---|---|
| AlsoKnownAs: | TEVprotease,NIa,Tobaccoetchvirusprotease |
| CatalogNo.: | P1001-10000 |
| Size: | 10X1mg |
| MolecularWeight: | 27kDa |
| Species: | TabaccoEtchVirus |
| Source: | Bacterialrecombinant |
| Stock: | 100mMTris,pH8.5at40C,500mMNaCl,5mMDTT,0.5mMEDTAand50%Glycerol |
| Concentration: | Seetubelabel |
| QualityAssurance: | >90%puritybasedonSDS-PAGE. |
| Storage: | Storeat-80°C;avoidmultiplefreeze-thawcycles |
| PDFDataSheet: | PDFDatasheet,MSDS |
| NCBIRefSeq: | N/A |
| Image(s): | ![]() |
| ShippingMethod: | Dryiceshipping |
| References: | 1.LucastLJ.,etal.(2001)Biotechniques30,544. |


