ubpbio/(Suc Leu Val Tyr)2-罗丹明110/G1110/2 mg
商品编号:
G1110
市场价:
¥2000.00
美元价:
1200.00
联系Q Q:
3392242852
电话号码:
4000-520-616
电子邮箱:
info@ebiomall.com
商品介绍
(Suc-LLVY)2-Rhodamineisafluorogenicsubstrateforthechymotrypsin–likeactivityofthe20Sand26Sproteasomes.Workingconcentrationsofthissubstrateis20-100µM.ThereleasedRhodamine110fluorescencecanbedetectedbyafluorimeterorplatereaderatexcitation/emissionof495nm/520nm,respectively.ComparedtoAMC,Rhodamine110isexcitedatlongerwavelengththatcansignificantlyenhancethesignal/noiseratio.ThissubstrateisextremlyvaluableforscreeningsmallchemicalinhibitorsofproteasomesbyavoidingtheinterferencefromchemicalcompoundautofluorescencethatoftenoccursatshorterwavelengthsaroundUVornear-UVregions.
Whenusedtodetermineproteasomeactivityincelllysates,celllysatesthatarepre-treatedwithaproteasomeinhibitorsuchasMG132,PS341orepoxomicincanbeusedtodeterminethefluorescencecontributedbyothercellularproteasesthatcleavethissubstrate.ReADIngsfromproteasomeinhibitor-treatedlysatesshouldbesubtracted.
Whenusedtodetermineproteasomeactivityincelllysates,celllysatesthatarepre-treatedwithaproteasomeinhibitorsuchasMG132,PS341orepoxomicincanbeusedtodeterminethefluorescencecontributedbyothercellularproteasesthatcleavethissubstrate.ReADIngsfromproteasomeinhibitor-treatedlysatesshouldbesubtracted.
AdditionalInformation
ProductName: | (Suc-Leu-Leu-Val-Tyr)2-Rhodamine110 |
---|---|
AlsoKnownAs: | (Suc-LLVY)2-Rhodamine110 |
CatalogNo.: | G1110 |
Size: | 2mg |
MolecularWeight: | 1507.7Da |
Species: | N/A |
Source: | Synthetic |
Stock: | Powder |
Concentration: | N/A |
QualityAssurance: | >98%byHPLCandNMR |
Storage: | EligIBLeforroomtemperatureshipping.Storeat-80°Cuponreceiving;avoidmultiplefreeze-thawcyclesafterdissolvinginDMSO. |
PDFDataSheet: | DownloadPDFdatasheet,MSDS |
NCBIRefSeq: | N/A |
Image(s): | No |
ShippingMethod: | Roomtemperatureshipping |
References: | No |