ubpbio/IPTG/P1010-500/5 X 100克
市场价:
¥27600.00
美元价:
16560.00
产品分类:
蛋白纯化试剂盒
公司分类:
Protein_purification_kit
联系Q Q:
3392242852
电话号码:
4000-520-616
电子邮箱:
info@ebiomall.com
商品介绍
IPTG,Isopropylβ-D-1-thiogalactopyranoside,bindsthelacrepressorandinducesexpressionofβ-galactosidaseinE.coli.IPTGiswidelyusedtoinducetheexpressionofclonedgenesunderthecontrolofthelacoperonandtoscreenblue/whitecolonyinX-galplate.
Note:Toprepare10mL1MIPTGstocksolution,weight2.38gIPTGanddissolvein8mldistilledwater,adjusttoafinalvolumeof10mlwithdistilledwater.Sterilethesolutionusinga0.22umsyringefilter.Aliquotthestockandstoreunder-20°Cforuptooneyear.
Note:Toprepare10mL1MIPTGstocksolution,weight2.38gIPTGanddissolvein8mldistilledwater,adjusttoafinalvolumeof10mlwithdistilledwater.Sterilethesolutionusinga0.22umsyringefilter.Aliquotthestockandstoreunder-20°Cforuptooneyear.
AdditionalInformation
| ProductName: | IPTG |
|---|---|
| AlsoKnownAs: | isopropyl-β-D-thiogalactopyranoside |
| CatalogNo.: | P1010-500 |
| Size: | 5X100g |
| CAS: | 367-93-1 |
| Formula: | C9H18O5S |
| MolecularWeight: | 238.3 |
| Form: | Powder |
| QualityAssurance: | >99%byHPLC,dioxane-free |
| Source: | Synthetic |
| Storage: | EligIBLeforambienttemperatureshipping.Storeat4°Cuponreceiving. |
| PDFDataSheet: | PDFDatasheet |
| Image(s): | ![]() |
| ShippingMethod: | Roomtemperatureshipping |
| References: | J.Sambrook,D.W.Russell,MolecularCloning:ALaboratoryManual,theThirdedition(ColdSpringHarborLaboratoryPress,ColdSpringHarbor,NewYork,A1.27,2001)pp.1.124-1.125. |


ExpressionofGST-UCHL3inBL21(DE3)cellswasinducedbytheadditionof25μM(lane3),100μM(lane4),200μM(lane5)or400μM(lane6)IPTGat370Cfor2hours.Lane2withoutIPTG.