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ubpbio/Ubiquitin (K0)/E1610/1 mg
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发布时间:2024-05-16
Ubiquitin (Ub) is a 76 amino acid protein widely expressed in the cytoplasmic and nucleus of cells. Ub is posttranslationally conjugated to proteins by the E1, E2, E3 protein ubiquitination cascade. Ub can be conjugated on proteins as monoUb or polyUb chains. Protein ubiquitination plays both proteolytic and nonproteolytic functions. Usually, polyubiquitinated proteins are targeted to the 26S proteasome for proteolysis. Typical concentration to support in vitro ubiquitination is 50-100 µM.All lysine residues in Ub(K0) were substituted with arginine residues, thereby Ub(K0) can only support monoubiquitination, but not polyubiquitination.
Additional Information
Ubiquitin (K0) |
UbK0 |
E1610 |
1 mg |
8.5 kDa |
Human |
Bacterial recombinant |
20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol |
See tube label |
~95% by SDS-PAGE, see datasheet for gel image |
Store at -80°C; avoid multiple freeze-thaw cycles |
PDF Datasheet, MSDS |
N/A |
No |
Dry ice shipping |
1. Hershko A, et al. (1980) Proc Natl Acad Sci USA 77(4), 1783 – 1786.2. Pickart CM (1997) FASEB 11(13), 1055 – 1066.3. Hershko A, et al. (1998) Ann Rev Biochem 67, 425 – 479.4. Jiang X, et al. (2012) Nature Reviews Immunology 12, 35 – 48.5. Bremm A, et al. (2012) Methods Mol Biol 832, 291 – 228. |
Details
Ubiquitin (Ub) is a 76 amino acid protein widely expressed in the cytoplasmic and nucleus of cells. Ub is posttranslationally conjugated to proteins by the E1, E2, E3 protein ubiquitination cascade. Ub can be conjugated on proteins as monoUb or polyUb chains. Protein ubiquitination plays both proteolytic and nonproteolytic functions. Usually, polyubiquitinated proteins are targeted to the 26S proteasome for proteolysis. Typical concentration to support in vitro ubiquitination is 50-100 µM.All lysine residues in Ub(K0) were substituted with arginine residues, thereby Ub(K0) can only support monoubiquitination, but not polyubiquitination.本文链接: http://ubpbio.immuno-online.com/view-1468993736.html
发布于 : 2024-05-16
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