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ubpbio/Anti-GFP/Y1031/100 µg
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发布时间：2024-05-16
Anti-GFP (GF28R) Mouse Monoclonal Antibody
![](\"https://www.ebiomall.com/images/ubpbio/Y1030_Anti-GFP_Tag.jpg\" "\\"Y1030image\\"")
1:1000 (1 μg/ml) Ab dilution probed against HEK293 cells transfected with GFP-tagged protein vector: untransfected control (Lane 1), 1μg (Lane 2) and 10μg (Lane 3) of cell lysates used
Additional Information
| Anti-GFP |
| Anti-GFP (GF28R) Mouse Monoclonal Antibody |
| Y1031 |
| 100 µg |
| ~55/25 kDa |
| N/A |
| Immunogen: GFP from the jellyfish Aequorea Victoria N-terminal peptide-KLH conjugates |
| 10 mM PBS (pH 7.2), 10% Glycerol, 0.09% NaN3 (sodium azide) |
| 1 µg/µl |
| WB (with ECL): 1-10 ng of purified GFP or GFP fusion proteins |
| Store at -20°C or -80°C for one year. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze-thaw cycles. |
| PDF Datasheet， MSDS |
| N/A |
| (Click image to enlarge)1:1000 (1 μg/ml) Ab dilution probed against HEK293 cells transfected with GFP-tagged protein vector: untransfected control (Lane 1), 1μg (Lane 2) and 10μg (Lane 3) of cell lysates used |
| Wet ice shipping |
| N/A |
Details
SpecificityRecognizes native and denatured forms of GFP and its variants such as: EGFP, YFP, EYFP, and CFPIsotypeMouse IgG2bCloneGF28RPurificationProtein A affinity chromatography from mouse ascites fluidApplicationsDot, ELISA, IP, IS, WBWorking ConditionsWB (with ECL): 1:1000-3000 dilutionIS: 1:500-2000 dilutionFor best results with other assays (e.g.: Dot, ELISA, IP, etc), please determine optimal working dilution by titration testImages(Click image to enlarge)1:1000 (1 μg/ml) Ab dilution probed against HEK293 cells transfected with GFP-tagged protein vector: untransfected control (Lane 1), 1μg (Lane 2) and 10μg (Lane 3) of cell lysates used本文链接： http://ubpbio.immuno-online.com/view-1500491527.html
发布于 ： 2024-05-16
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