ubpbio/6xHis-Ubiquitin (K63 only)/E1880/1 mg

来自 : 发布时间：2024-05-16

Ubiquitin (Ub) is a 76 amino acid protein widely expressed in the cytoplasmic and nucleus of cells. Ub is posttranslationally conjugated to proteins by the E1, E2, E3 protein ubiquitination cascade. Ub can be conjugated on proteins as monoUb or polyUb chains. Protein ubiquitination plays both proteolytic and nonproteolytic functions. Usually, polyubiquitinated proteins are targeted to the 26S proteasome for proteolysis. Typical concentration to support in vitro ubiquitination is 50-100 µM. In Ub(K63 only), all lysine residues but lysine 63 were substituted with arginines, thereby it only supports the formation of lysine 63-linked polyubiquitin chains.

Additional Information

Product Name: Also Known As: Catalog No.: Size: Molecular Weight: Species: Source: Stock: Concentration: Quality Assurance: Storage: PDF Data Sheet: NCBI RefSeq: Image(s): Shipping Method: References:

6xHis-Ubiquitin (K63 only)

6xHis-[K63-only]Ub

E1880

1 mg

8.5 kDa

Human

Bacterial recombinant

20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol

See tube label

~95% by SDS-PAGE, see datasheet for gel image

Store at -80°C; avoid multiple freeze-thaw cycles

PDF Datasheet， MSDS

N/A

No

Dry ice shipping

1. Hershko A, et al. (1980) Proc Natl Acad Sci USA 77(4), 1783 – 1786.2. Pickart CM (1997) FASEB 11(13), 1055 – 1066.3. Hershko A, et al. (1998) Ann Rev Biochem 67, 425 – 479.4. Jiang X, et al. (2012) Nature Reviews Immunology 12, 35 – 48.5. Bremm A, et al. (2012) Methods Mol Biol 832, 291 – 228.

Details

Ubiquitin (Ub) is a 76 amino acid protein widely expressed in the cytoplasmic and nucleus of cells. Ub is posttranslationally conjugated to proteins by the E1, E2, E3 protein ubiquitination cascade. Ub can be conjugated on proteins as monoUb or polyUb chains. Protein ubiquitination plays both proteolytic and nonproteolytic functions. Usually, polyubiquitinated proteins are targeted to the 26S proteasome for proteolysis. Typical concentration to support in vitro ubiquitination is 50-100 µM. In Ub(K63 only), all lysine residues but lysine 63 were substituted with arginines, thereby it only supports the formation of lysine 63-linked polyubiquitin chains.Images(Click image to enlarge)Coomassie-stained SDS-PAGELane 1: Molecular weight markersLane 2: 5 µg purified 6xHis-Ubiquitin (K0)Lane 3: 5 µg purified 6xHis-Ubiquitin (K6 only)Lane 4: 5 µg purified 6xHis-Ubiquitin (K11 only)Lane 5: 5 µg purified 6xHis-Ubiquitin (K27 only)Lane 6: 5 µg purified 6xHis-Ubiquitin (K29 only)Lane 7: 5 µg purified 6xHis-Ubiquitin (K33 only)Lane 8: 5 µg purified 6xHis-Ubiquitin (K48 only)Lane 9: 5 µg purified 6xHis-Ubiquitin (K63 only)