ubpbio/GST-NEMO (UBAN)/I1530/100 µg

来自 : 发布时间：2024-05-15

GST was fused on the N-terminus of the linear polyubiquitin chain binding domain of NEMO en-compassing amino acids 183-339. This fusion protein can be used for in vitro GST pulldown as-says and for enrichment of cellular proteins conjugated with linear polyubiquitin chains in whole cell or tissue lysates. GST-NEMO (UBAN) can be precipitated using glutathione resin. After washing, GST-NEMO (UBAN)and its bound proteins can be eluted by a buffer containing 10 mM glutathione.

Additional Information

Product Name: Also Known As: Catalog No.: Size: Molecular Weight: Species: Source: Stock: Concentration: Quality Assurance: Storage: PDF Data Sheet: NCBI RefSeq: Image(s): Shipping Method: References:

GST-NEMO (UBAN)

IKBKG; IP; IP1; IP2; FIP3; IPD2; NEMO; FIP-3; Fip3p; AMCBX1; ZC2HC9; IKK-gamma

I1530

100 µg

18.4 kDa

Human

Bacterial recombinant

20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol

See tube label

~90% by SDS-PAGE, see datasheet for gel image

Store at -80°C; avoid multiple freeze-thaw cycles

PDF Datasheet， MSDS

NM_001099857

(Click image to enlarge)

Coomassie-stained SDS-PAGELane 1: Molecular weight markersLane 2: 5 µg purified GST-NEMO (UBAN)

Dry ice shipping

1. van Wijk SJ, et al. (2013) Nat Protoc. 8(7):1449-582. van Wijk SJ, et al. (2012) Mol Cell. 14;47(5):797-809

Details

GST was fused on the N-terminus of the linear polyubiquitin chain binding domain of NEMO en-compassing amino acids 183-339. This fusion protein can be used for in vitro GST pulldown as-says and for enrichment of cellular proteins conjugated with linear polyubiquitin chains in whole cell or tissue lysates. GST-NEMO (UBAN) can be precipitated using glutathione resin. After washing, GST-NEMO (UBAN)and its bound proteins can be eluted by a buffer containing 10 mM glutathione.Images:(Click image to enlarge) Coomassie-stained SDS-PAGELane 1: Molecular weight markersLane 2: 5 µg purified GST-NEMO (UBAN) Note:1. NEMO(183-339) may also bind long polyubiquitin chains linked via lysine 63. 2. Dissolve 10 mM glutathione in a neutral buffer could drop pH to 3-4. Use 0.2 M NaOH to ad-just pH back to neutral.